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PEDF maintains endothelial tight junction stability through 67LR. (A–C) Transfection with both 67LR and PEDFR viruses was successful. (D,E) Western blotting to detect the effect of PEDF on the expression of ZO-1 in vitro; (F) Immunofluorescence to observe the effect of PEDF on the distribution of ZO-1 in vitro. Scale Bar = 200 μm. Data are expressed as mean ± SD; n = 6; NS, no significant difference; * p < 0.05; *** p < 0.001.
