| Antibody |
5D3 (mouse monoclonal) |
Hybridoma was donated by Brian P. Sorrentino |
|
Prepared from hybridoma supernatant by affinity chromatography |
| Antibody |
BXP-21 (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-58222 |
(1:2500 dilution) |
| Antibody |
C-2 (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-8432 |
(1:2500 dilution) |
| Antibody |
Goat anti-mouse IgG-HRP (polyclonal) |
Santa Cruz Biotechnology |
Cat# sc-2005 |
(1:2500 dilution) |
| Cell line (Canis familiaris, dog) |
MDCK II, (epithelial-like cells from kidney distal tubule) |
Obtained from Prof. Gerrit van Meer |
ECACC 00062107 |
Mycoplasma-free |
| Cell line (Mus musculus, mouse) |
5D3 hybridoma |
Donated by Brian P. Sorrentino |
|
Mycoplasma-free |
| Chemical compound, drug |
Mitoxantrone |
Sigma-Aldrich |
Cat# M6545 |
|
| Chemical compound, drug |
Ko143 |
Sigma-Aldrich |
Cat# K2145 |
|
| Chemical compound, drug |
Quercetin |
Sigma-Aldrich |
Cat# Q4951 |
|
| Chemical compound, drug |
Estrone-3-sulfate |
Sigma-Aldrich |
Cat# E9145 |
|
| Chemical compound, drug |
ADP |
Sigma-Aldrich |
Cat# A2754 |
|
| Chemical compound, drug |
AMP-PNP |
Sigma-Aldrich |
Cat# A2647 |
|
| Chemical compound, drug |
ATP |
Sigma-Aldrich |
Cat# A2383 |
|
| Chemical compound, drug |
Sodium-orthovanadate |
Sigma-Aldrich |
Cat# S6508 |
|
| Chemical compound, drug |
Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat# P2714 |
|
| Chemical compound, drug |
DL-Dithiothreitol |
Sigma-Aldrich |
Cat# D0632 |
|
| Software, algorithm |
Flowing software |
Turku Centre for Biotechnology |
|
https://bioscience.fi/services/cell-imaging/flowing-software/
|
| Software, algorithm |
MATLAB |
Mathworks Inc. |
|
https://www.mathworks.com/products/matlab.html
|
| Software, algorithm |
QuickFit 3.0 |
|
|
https://biii.eu/quickfit-3
|
| Software, algorithm |
SigmaPlot |
Systat Software Inc. |
|
https://systatsoftware.com/sigmaplot/
|
| Other |
Eight-well chambered coverslip plate |
ibidi GmbH |
Cat# 80826-90 |
Imaging chamber for microscopy |
| Other |
Streptolysin-O (SLO) from Streptococcus pyogenes |
Sigma-Aldrich |
Cat# S5265 |
(250 U/ml) |
| Other |
Alexa 647 succinimidyl ester |
Life Technologies Inc. |
Cat# A20006 |
Fluorescent dye |
| Other |
Propidium iodide |
Sigma-Aldrich |
Cat# P4170 |
Fluorescent dye |
| Other |
SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Fisher Scientific |
Cat# 34579 |
Reagent for protein detection in western blot analysis |
