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. 2023 Feb 3;24(3):3028. doi: 10.3390/ijms24033028

Figure 2.

Figure 2

TFEB knockdown exacerbates HK-2 cells damage caused by cisplatin. (A) Western blot assay of TFEB expression in HK-2 cells with or without TFEB knockdown. (B) HK-2 cells with or without TFEB knockdown were exposed to cisplatin (20 μM) for 24 h. The CCK-8 assay was used to detect cell viability. (C,D) Flow cytometry and quantitative analysis of apoptosis in HK-2 cells. Each bar represents mean ± SEM of at least three independent experiments. siTFEB: TFEB-siRNA; Cis: cisplatin. * p < 0.05, *** p < 0.001, NS, non significant.