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. 2023 Feb 3;24(3):3028. doi: 10.3390/ijms24033028

Figure 4.

Figure 4

TFEB knockdown aggravates cisplatin-induced mitochondrial damage in HK-2 cells. HK-2 cells with or without TFEB knockdown were exposed to cisplatin (20 μM) for 24 h. (A,B) Flow cytometry analysis of TMRE fluorescence intensity to detect mitochondrial membrane potential in HK-2 cells. (C) Immunofluorescence staining with MitoTracker to detect mitochondrial morphology in HK-2 cells treated as indicated. Scale bar: 10 μm. (D) Percentage of cells with fragmented mitochondria in HK-2 cells. Each bar represents mean ± SEM of at least three independent experiments. siTFEB: TFEB-siRNA; Cis: cisplatin. * p < 0.05, ** p < 0.01, *** p < 0.001.