Figure 6.

Effect of TFEB overexpression on ROS and MitoSOX in cisplatin-induced HK-2 cells. (A,B) Flow cytometry analysis and quantitative data of ROS in HK-2 cells infected by lentivirus carrying with or without TFEB followed by cisplatin (20 μM) exposure for 24 h. (C,D) Mitochondrial ROS (mtROS) were measured by incubation with MitoSOX in HK-2 cells with indicated treatments. Each bar represents the mean ± SEM for at least three independent experiments. OE-TFEB: overexpressing TFEB; Cis: cisplatin. * p < 0.05, ** p < 0.01, *** p < 0.001.