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. 2023 Feb 3;24(3):3047. doi: 10.3390/ijms24033047

Figure 5.

Figure 5

CASC19 positively regulated the expression of FKBP5 through miR-340-3p: (A,B) RT-qPCR (A) and Western blot (B) assay of the expression of FKBP5 in CNE2R cells transfected with miR-340-3p mimics or its control; (C) prediction of the target binding sites of FKBP5 and miR-340-3p was verified with the dual-luciferase reporter assay; (D) the expression of FKBP5 in CNE2R cells with or without transfection of siCASC19; (E) the expression of CACS19 in CNE2R cells with or without transfection of siFKBP5; (F) Western blot assay of FKBP5, P62, and LC3 protein in CNE2R cells co-transfected with siCASC19 and FKBP5 overexpression plasmid. * p < 0.05 compared with the corresponding control.