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. 2023 Feb 3;24(3):3019. doi: 10.3390/ijms24033019

Figure 2.

Figure 2

Characterization of corrected iPSCs from GIT patients (CMC-GIT-001corr). (A) Morphology of corrected GIT patient iPSCs (CMC-GIT-001corr). (B) Flow cytometry analysis of cells expressing NANOG, SSEA-4, and TRA-1-81. (C) Representative immunofluorescence image of pluripotency markers NANOG, SSEA-4, and TRA-1-81. (D) Immunofluorescence staining of three germ layer markers. Ectoderm, mesoderm, and entoderm differentiation were detected by PAX4, SM22a, and FOX2A, respectively. Scale bar = 50 μm. (E) Chromosome karyotyping of CMC-GIT-001corr hiPSCs. (F) Mycoplasma detection by PCR, negative.