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. 2023 Feb 2;24(3):2841. doi: 10.3390/ijms24032841

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of 5mC and 5hmC expression in the human cornea. Representative immunostaining analyses of: (A) 5mC expression in the limbus (top) and central cornea (bottom), (B) ABCB5 and 5hmC co-expression in the limbus (top), and MUC16 and 5hmC co-expression in the central cornea (bottom), (C) TET1 and 5hmC co-staining in the limbus (top) and central cornea (bottom), (D) Co-expression of ABCB5 and TET3 in the limbus (top), and co-staining of MUC16 and TET3 in the central cornea (bottom), (E) ABCB5 and TET2 co-staining in the limbus (top), and MUC16 and TET2 co-expression in the central cornea (bottom), (F) Co-staining of H3S10ph and 5hmC in the limbus (top) and the central cornea (bottom). Nuclei stained with Hoechst 33342 (blue), n = 3. Scale bar, 20 µm.