Figure 3.

The effects of EGFR inhibitors on head-and-neck-cancer-cell viability. Cell lines were treated with afatinib or erlotinib at different concentrations for 72 h and cell viability was determined by MTT assay. The IC₅₀ curves of the effects of the inhibitors on Detroit 562 (A), FaDu (B) and SCC25 (C) cell lines are shown. The IC₅₀ curves of the effects of the afatinib on the three HNSCC cell lines are shown in a common diagram (D) for better comparison. (E) The IC₅₀ values for the effects of the EGFR inhibitors on Detroit 562, FaDu and SCC25 cells. All data are represented as the mean ± SD of five independent measurements. The IC₅₀ values (E) of the two inhibitors were compared to each other and Student’s t-tests were performed for statistically significant differences; * p < 0.05 afatinib vs. erlotinib and one afatinib-treated cell line vs. another.