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. 2023 Feb 1;24(3):2782. doi: 10.3390/ijms24032782

Figure 6.

Figure 6

Protein expression and phosphorylation in head-and-neck squamous cell carcinoma (HNSCC) cell lines. (A) Cells were subjected to Western blot analysis with antibodies against pY1068-EGFR, EGFR, pS473-Akt, Akt, pT202/Y204-ERK, ERK and the loading control, α-tubulin. (B) Densitometry analysis of pY1068-EGFR, EGFR, pS473-Akt, Akt, pT202/Y204-ERK and ERK expression in Detroit 562, FaDu and SCC25 cells. The expressions of all proteins were normalized to expression of α-tubulin. All data are presented as mean ± SD of three independent experiments. The pY1068-EGFR, EGFR, pS473-Akt, Akt, pT202/Y204-ERK and ERK levels of the cell lines were compared to each other, and Student’s t-tests were performed for statistically significant differences; * p < 0.05 one cell line vs. another.