Figure 1.

Identification of HNE-modified proteins from erythrocyte membrane of HH patients. (A) Western blots of HH patients (P1–P6) and healthy controls (C1 and C2). Protein spots identified by MALDI-TOF-TOF are designated with “a–m” letters and encircled with colours: red are spots present in patients and controls; blue spots are present only in HH patients; and yellow spots are only in controls. (B) Representative two-dimensional Coomassie brilliant blue-stained gel with total erythrocyte membrane proteins (Control sample 1). 4-hydroxynonenal (HNE), Hereditary hemochromatosis (HH).