Figure 3.

HNE-modified proteins in organs from female and male Hfe^−/− mice. Homogenates from each organ were separately resolved on 12% polyacrylamide gels. HNE (4-hydroxynonenal) modified proteins were detected by Western blot using HNE specific antibodies. (A) Western blot analysis of HNE adducts in kidney, brain, and heart proteins from female and male control (C) and Hfe^−/− (H) mice at 3, 5 and 7 months of age. Images are representative of n = 3 per group. (B) Coomassie brilliant blue-stained gels where HNE-immunoreactive bands protein bands were excised. Arrows indicate the protein bands that were excised from the gel and the molecular weight are expressed in KDa on the left side of the arrow.