Figure 2.

Overexpression of hXIAP reduces neuronal death after SCI. (A) Representative pictures obtained after processing of stained sections with TruAI integrated cellSens Dimensions software for neuron counting. (B) Estimations of the number of neurons in transverse sections comprising 4.8 mm surrounding the injury epicentre of the spinal cord show that CB57^hXIAP+ mouse sections (red symbols) have a significant increase in neuron counts with respect to CB57^WT mouse sections (blue symbols), particularly in sections caudal to the injury epicentre (symbols represent mean ± SEM and line the polynomial non-linear regression adjusts; * = p-value < 0.05 and ** = p-value < 0.01 for injured CB57^hXIAP+ vs. CB57^WT mice; in Student’s t-test; †† = p-value < 0.01 and ††† = p-value < 0.001 for injured CB57^WT mice vs. sham animals (dash-dotted line = sham CB57^WT; dotted line = CB57^hXIAP+); n = 3–6 animals/group).