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Overview of the VASA-seq workflow. (A) Collection of RNA in single cells. (B) Fragmentation process of RNA fragments. (C) Repair of RNA fragments and polyadenylation. (D) Reverse transcription of mRNA and completion of cDNA synthesis (E) In vitro transcription process. (F) Adaptation of aptamer Ligation and reverse transcription processes. (G) Indexing PCR.
