Table 1.
Comparison of different ScRNA-Seq technology approaches.
| Platform Name | Separation Method | Amplification Method | Using UMI | Amplification Range | Advantages | Disadvantages | Release Date | References |
|---|---|---|---|---|---|---|---|---|
| VASA-seq | FANS | PCR | YES | All transcripts | Low cost and accurate dosing | / | 2022 | [10] |
| Smart-seq3 | Microfluidics | PCR | YES | 5′ end | High sensitivity | Time-consuming | 2020 | [11,12] |
| DNBelabC4 | Microfluidics | PCR | YES | All transcripts | Precise quantification | / | 2019 | [13] |
| Seq-Well | Microfluidics | PCR | YES | 3′ end | Low cost and precise quantification | Unsuitable for variable splicing and allelic expression | 2017 | [14] |
| MATQ-seq | FACS | PCR | YES | All transcripts | Precise quantification | Low cell throughput | 2017 | [15] |
| 10× Genomics | Microfluidics | PCR | YES | 3′ end | High cell capture efficiency, fast cycle time, high cell suitability, and reproducibility | Sequencing can be performed only for the 3′ end | 2016 | [16] |
| Cyto-Seq | Microfluidics | PCR | YES | 3′ end | Low cost and high throughput | Cross-contamination between RNAs | 2015 | [17] |
| SC3-seq | Micromanipulation | PCR | YES | 3′ end | Good reproducibility and accurate quantification | Recognize DNA at the 3′ end | 2015 | [18] |
| inDrop-seq | Microfluidics | IVT | YES | 3′ end | Low cost and linear amplification | Long operating time and high initial cell concentration | 2015 | [19] |
| Drop-seq | Microfluidics | PCR | YES | 3′ end | Low cost and high throughput | Low cell capture rate | 2015 | [20] |
| MARS-seq | FACS | IVT | YES | 3′ end | High specificity | Low amplification efficiency | 2014 | [21] |
| STRT-seq | Microfluidics | PCR | NO | All transcripts | Accurate positioning of transcripts at the 5′ end to reduce amplification bias | Low sensitivity, only available for identification of 5′ end DNA | 2014 | [22,23] |
| Quartz-seq | FACS | PCR | YES | 3′ end | High sensitivity, reproducibility, and operational simplicity | Higher noise levels | 2013 | [24] |
| Fluidigm C1 | Microfluidics | PCR | NO | All transcripts | Simple process | High cost and low throughput | 2013 | [25] |
| Smart-seq2 | FACS | PCR | NO | All transcripts | Full-length cDNA detects structural and RNA shear variants | High cost, low throughput, and time-consuming | 2013 | [13,26] |
| Smart-seq | FACS | PCR | NO | All transcripts | High sensitivity to reduce the rates of nucleic acid loss | Low throughput and the existence of transcript length bias | 2012 | [22] |
| CEL-seq | FACS | IVT | YES | 3′ end | Good reproducibility and highly sensitive | Low throughput and amplification efficiency, library biased toward the 3′ end of the gene | 2012 | [27] |
| Tang-2009 | FACS | PCR | NO | 3′ end | Good reproducibility | High cost and low throughput | 2009 | [9] |