Table 2.
Latest applications of ScRNA-Seq in tissue and organ research.
| Tissues and Organs | Methods | Stromal Cell Subtypes | Key Difference Genes | Mechanisms | References |
|---|---|---|---|---|---|
| Liver | 10× Genomics | ID3+ hepatocytes NCAM1+ cholangiocytes Sox9+ cholangiocytes |
ID3 | Inhibition of the Wnt signaling pathway maintains ID3+ cells in an undifferentiated hepatocyte-like state. | [85] |
| COL1A1 | |||||
| HAND2 | |||||
| Brain | 10× Genomics | Astrocyte Radial glia cell BMP single-related cells |
CLIC6 | Pathways associated with metabolic stress, such as glycolysis, ER stress, and hypoxia, are significantly less activated in IVD-like organs. | [86] |
| ZBTB20 | |||||
| STRN3 | |||||
| Heart | STAR | Valvular interstitial cells Cardiac fibroblasts Endothelial cells |
HAND1 | Endocardial cells highly express ligands and receptors of the Notch signaling pathway, which regulates neuromodulin (NRG)/ERBB signaling to promote cardiomyocyte differentiation from the trabecular layer. | [87] |
| HEY2 | |||||
| IRX3 | |||||
| Kidney | 10× Genomics | CDH1+/JAG1+ cells JAG1+/Jag1+ cells |
SLC39A8 | Notch pathways in JAG1 and HES1-expressing proximal/medial renal vesicles are tightly linked. | [88] |
| LAMP5 | |||||
| HNF4A | |||||
| Pharynx | 10× Genomics | Parathyroid mTEC cTEC |
Irf628 | Hippo signaling is active in the developing thymus, but absent in the Foxn1 thymus, suggesting that this pathway may function downstream of Foxn1. | [89] |
| Trp63 | |||||
| Vim29 | |||||
| Ovary | 10× Genomics | Vascular smooth muscle cells Ovarian luminal epithelial cells Stromal progenitor cells |
Foxl2l | Inhibition of BMP and Wnt signaling pathways can keep prefollicular cells in an undifferentiated stem cell-like state. | [90] |
| Wnt9b | |||||
| Nanos2 |