Table 3.
Statistically significant brain N-glycans by week 12 in the three different pairs of conditions: HFD vs. CTRL, MED vs. CTRL, and MED vs. HFD. HFD, high-fat diet; CTRL, normal-fat diet; MED, Mediterranean-like diet; FC, fold change.
| HFD vs. CTRL | |||||
|---|---|---|---|---|---|
| Glycan Name 1 | Composition 2 | Glycan Mass | m/z | p | FC |
| F(6)A2BG(4)2S(6,6)2 | NeuAc2 Gal2 Fuc1 GlcNAc5 Man3 | 2571.92 | 1442.5620 | 0.0126 | −1.20 |
| A2[6]G(4)1 | Gal1 GlcNAc4 Man3 | 1478.54 | 895.8720 | 0.0069 | 1.19 |
| - | HexNAc4 Hex5 NeuAc3 | 2513.87 | 1414.0499 | 0.0046 | −1.28 |
| F(6)A2[3]BG(4)1 | Gal1 Fuc1 GlcNAc5 Man3 | 1827.68 | 1070.4368 | 0.0426 | 1.10 |
| - | HexNAc6 Hex5 Fuc1 NeuAc3 | 3066.0951 | 1126.7695 | 0.0236 | 1.35 |
| M11 a3D1,[D2(1),D3(1)],a2D4(2) | GlcNAc2 Man11 | 2206.75 | 1259.9943 | 0.0036 | −1.32 |
| F(6)A2[6]G1Ga1 | Fuc1 Gal2 GlcNAc4 Man3 | 1786.65 | 1049.9261 | 0.0038 | 1.15 |
| F(6)A2G(4)2S(6,6)2 | NeuAc2 Gal2 Fuc1 GlcNAc4 Man3 | 2368.84 | 1341.0211 | 0.0266 | −1.23 |
| F(6)A2G(4)2S(3,3)2 | NeuAc2 Gal2 Fuc1 GlcNAc4 Man3 | 2368.84 | 1341.0211 | 0.0215 | −1.25 |
| M6 D1 | GlcNAc2 Man6 | 1396.49 | 854.8422 | 0.0050 | 1.12 |
| - | HexNAc6 Hex5 Fuc2 | 2338.8667 | 1325.9973 | 0.0103 | −1.54 |
| - | HexNAc5 Hex6 NeuAc3 | 2879.01 | 1064.7489 | 0.0366 | −1.16 |
| - | HexNAc6 Hex3 Fuc1 NeuAc2 | 2450.8940 | 1382.0477 | 0.0146 | −1.23 |
| - | HexNAc8 Hex7 | 2777.0153 | 1029.7350 | 0.0059 | −1.24 |
| MED vs. CTRL | |||||
| Glycan Name | Composition | Glycan Mass | m/z | p | FC |
| F(6)A2BG(4)2S(6,6)2 | NeuAc2 Gal2 Fuc1 GlcNAc5 Man3 | 2571.92 | 1442.5621 | 0.0478 | −1.22 |
| M7 | GlcNAc2 Man7 | 1558.54 | 935.8692 | 0.0310 | 1.15 |
| - | HexNAc4 Hex5 NeuAc3 | 2513.87 | 1414.0499 | 0.0233 | −1.29 |
| F(6)A2[3]BG(4)1 | Gal1 Fuc1 GlcNAc5 Man3 | 1827.68 | 1070.4368 | 0.0007 | 1.19 |
| F(6)A2G(4)2S(6,6)2 | NeuAc2 Gal2 Fuc1 GlcNAc4 Man3 | 2368.84 | 1341.0211 | 0.0300 | −1.26 |
| - | HexNAc6 Hex6 Fuc1 NeuAc3 | 3228.15 | 1180.7875 | 0.0226 | −1.41 |
| F(6)A3G(4)3S(3,3,3)3 | NeuAc3 Gal3 Fuc1 GlcNAc5 Man3 | 1915.69 | 1113.0953 | 0.0328 | −1.50 |
| - | HexNAc6 Hex7 NeuAc4 | 3535.24 | 1283.491 | 0.0495 | −1.30 |
| F(6)A2[6]G1Ga1 | Fuc1 Gal2 GlcNAc4 Man3 | 1786.65 | 1049.9261 | 0.0073 | 1.11 |
| MED vs. HFD | |||||
| Glycan Name | Composition | Glycan Mass | m/z | p | FC |
| - | HexNAc6 Hex5 Fuc1 NeuAc3 | 3066.0951 | 1126.7695 | 0.0285 | −1.59 |
1 Employed glycan nomenclature: F—Fucose; G—Galactose; S—N-Acetylneuraminic acid; Ga—a-linked Galactose; A1—Monoantennary, A2—Biantennary, B, bisecting GlcNAc linked α1-4 to α1-3 mannose. Numbers with parentheses indicate the preceding monosaccharide’s linkage while those not in parentheses indicate the preceding characteristic’s number. For example, F(6)A3G(4)3S(3,3,3)3 represents a core fucosylated triantennary glycan with three galactoses directly attached to antennae, and the three antennae terminated with an N-glycolylneuraminic acid. 2 Employed glycan nomenclature for glycan composition: HexNAc—N-Acetylhexosamine; Hex—Hexose; NeuAc—N-Acetylneuraminic acid; Fuc—Fucose; Gal—Galactose; GlcNAc—N-Acetylglucosamine; Man—Mannose.