Figure 4.

Single-cell sorting strategy of the SIN3A bi-allelically tagged cells

(A and B) Cells were separated from the debris of various sizes based on the forward scatter area (FSC-A) and side scatter area (SSC-A) (Gata 1: SSC-A versus FSC-A). Then, single cells were separated using a single cell gate based on the width and area metrics of the forward scatter (Gate 2: FSC-W verse FSC-A) and side scatter (Gate 3: SSC-W versus SSC-A). Further, the gata for EGFP and mCherry negative cells were set using control cells without nucleofection (Gate 4: EGFP versus mCherry). Four cell populations were observed in the SIN3A nucleofection cell pool compared to the control cells. The EGFP and mCherry double positive population contains the cells with allelic tagging for two alleles of SIN3A.