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. 2023 Feb 1;4(1):102084. doi: 10.1016/j.xpro.2023.102084
Steps Temperature Time Cycles
Initial Denaturation 95°C 2 min 1
Denaturation 95°C 30 s 32
Annealing 55°C 30 s
Extension 72°C 1 min
Final extension 72°C 5 min 1
Hold 4°C Forever