| Tube 1: Cas9/sgRNA complex | Tube 2: Donor plasmids | ||
|---|---|---|---|
| Cas9-NLS protein | 16 μg | EGFP donor | 3 μg |
| sgRNA 1 | 4 μg | mCherry donor | 3 μg |
| sgRNA 2 | 4 μg | ||
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