Reagent | Final concentration | Amount |
---|---|---|
0.5 M EDTA | 2 mM | 0.2 mL |
1 M HEPES pH 7.0 | 25 mM | 1.25 mL |
Fetal Bovine Serum | 1% | 0.5 mL |
Penicillin-Streptomycin | 1% | 0.5 mL |
DPBS | N/A | 47.55 mL |
Total | N/A | 50 mL |
Reagent | Final concentration | Amount |
---|---|---|
0.5 M EDTA | 2 mM | 0.2 mL |
1 M HEPES pH 7.0 | 25 mM | 1.25 mL |
Fetal Bovine Serum | 1% | 0.5 mL |
Penicillin-Streptomycin | 1% | 0.5 mL |
DPBS | N/A | 47.55 mL |
Total | N/A | 50 mL |