| Component | Amount (50 μL reaction) |
|---|---|
| 20 μM forward primer | 0.5 μL |
| 20 μM reverse primer | 0.5 μL |
| Genomic DNA (200 ng) | Variable |
| NEBNext High-Fidelity 2× PCR Master Mix | 25 μL |
| Nuclease-free water | To 50 μL |
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