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. 2023 Feb 1;4(1):102084. doi: 10.1016/j.xpro.2023.102084
Component Amount
EGFP / mCherry empty donor fragment 50 ng
Left homology arm fragment 2–5 fold molar ratio to donor fragment
NEBuilder HiFi DNA Assembly Master Mix 10 μL
Nuclease-free water To 20 μL