Table 2.
Primers used for real-time PCR analysis.
| Gene | Primer | Sequence, 5′–3′ | Product size, bp |
|---|---|---|---|
| β-actin | Forward | AGTTGAAGGTGGTCTCGTGG | 216 |
| Reverse | TGCGGGACATCAAGGAGAAG | ||
| Slc6a14 | Forward | GTGGCTTGGGGTGGTTTAGT | 203 |
| Reverse | AACGCCAAATCGAAACCTGA | ||
| Slc36a1 | Forward | GGTGGAGATGGCAAGGGTT | 256 |
| Reverse | TCAGGGTCTGGAACCATGTTG | ||
| Slc2a2 | Forward | AAGTCGAGGCCTATGATCTGACTAA | 161 |
| Reverse | GGAAGAGGCATATCAGGACTCTACT | ||
| Slc6a1 | Forward | CAACTCCTTCACCACAACGC | 249 |
| Reverse | CAGCCAACACCCTTCCAGAT | ||
| Slc7a9 | Forward | GAAGAAGCCTCCTAGAAGTG | 268 |
| Reverse | CCAGTGTCGCAAGAATCC | ||
| SOD | Forward | GGTGAACCAGTTGTGTTGTCAGG | 114 |
| Reverse | ATGAGGTCCTGCACTGGTACAG | ||
| GPX | Forward | CGCTCTTTACCTTCCTGCGGAA | 120 |
| Reverse | AGTTCCAGGCAATGTCGTTGCG | ||
| CAT | Forward | TGCCCTTGCACAAACAAAACC | 254 |
| Reverse | GCTCAAACACCTTCGCCTTC | ||
| IL-1β | Forward | CCTGGACCTTGGTTCTCT | 123 |
| Reverse | GGATTCTTCATCGGCTTCT | ||
| IL-6 | Forward | GGCAAAAGGGAAAGAATCCAG | 87 |
| Reverse | CGTTCTGTGACTGCAGCTTATCC | ||
| IL-10 | Forward | GGGCTATTTGTCCTGACTGC | 105 |
| Reverse | GGGCTCCCTAGTTTCTCTTCC | ||
| IL-22 | Forward | AGCAAGCGTGAAGGTGCGGTT | 169 |
| Reverse | GCGGACATCTGGGAGCCCTTT | ||
| TNF-α | Forward | ACAGGCCAGCTCCCTCTTAT | 102 |
| Reverse | CCTCGCCCTCCTGAATAAAT | ||
| IFN-γ | Forward | CCATTCAAAGGAGCATGGAT | 146 |
| Reverse | GAGTTCACTGATGGCTTTGC | ||
| TGF-β | Forward | CGAGCCCTGGATACCAACTA | 164 |
| Reverse | AGGCTCCAGATGTAGGGACA | ||
| Muc2 | Forward | AGACGGGCGGAGACTTTGAATC | 102 |
| Reverse | CTTGGATGGGAACGCTGGGATA | ||
| Muc4 | Forward | TTGAAGGCTGGAGATTGCAGAGTC | 113 |
| Reverse | CATTAGCTCATACAGGGCACAGAAGG | ||
| PBD1 | Forward | CCTCCTCCTTGTATTCCTCCTC | 141 |
| Reverse | GTGCCGATCTGTTTCATCTTTG | ||
| PBD2 | Forward | CCTTGTATTCCTCCTCATGGTCC | 136 |
| Reverse | GGTGCCGATCTGTTTCATCTTTG | ||
| TLR2 | Forward | GTTCACGCATTTCCGCAGTT | 224 |
| Reverse | CTTTGTGGACAGCATGGGTCTT | ||
| TLR4 | Forward | AGCACCTATGACGCCTTTGTTA | 229 |
| Reverse | TACACCATCGGCTCTGTATGAA | ||
| TLR5 | Forward | TTAAGCCTTGCGGATAATAACCTGT | 213 |
| Reverse | GAAGCCAAACCCAGAACCCATA | ||
| Claudin-1 | Forward | CTAGTGATGAGGCAGATGAA | 250 |
| Reverse | AGATAGGTCCGAAGCAGAT | ||
| Occludin | Forward | GAGTGATTCGGATTCTGTCT | 181 |
| Reverse | TAGCCATAACCATAGCCATAG | ||
| ZO-1 | Forward | TTGATAGTGGCGTTGACA | 126 |
| Reverse | CCTCATCTTCATCATCTTCTAC |
β-actin = actin, beta 2; Slc6a14 = solute carrier family 6 member 14; Slc36a1 = solute carrier family 36 member 1; Slc2a2 = solute carrier family 2 member 2; Slc6a1 = solute carrier family 6 member 1; Slc7a9 = solute carrier family 7 member 1; SOD = superoxide dismutase; GPX = glutathione peroxidase; CAT = chloramphenicol acetyltransferase; IL-1β = interleukin-1β; IL-6 = interleukin-6; IL-10 = interleukin-10; IL-22 = interleukin-22; TNF-α = tumor necrosis factor-α; IFN-γ = interferon-γ; TGF-β = transforming growth factor-β; Muc2 = mucin 2; Muc4 = mucin 4; PBD1 = porcine β-defensin-1; PBD2 = porcine β-defensin-2; TLR2 = toll like receptor 2; TLR4 = toll like receptor 2; TLR5 = toll like receptor; ZO-1 = zonula occludens-1.