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. 2022 Dec 20;42(7):530–540. doi: 10.1038/s41388-022-02569-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Bar plot for the differential abundant species identified by LEfSe. Red bars stand for the enriched species in appendectomy patients, while blue bars represent the depleted species in appendectomy subjects. Species that were reported to promote cancer were marked in bold red fonts, while the cancer-inhibiting species were marked in bold green fonts. B The relative abundance for representative appendectomy enriched species, Bacteroides vulgatus and Bacteroides fragilis; and appendectomy-depleted species, Blautia sp SC05B48 and Collinsella aerofaciens in all subjects, young and old subgroups are compared and shown as box plot. C The validation of identified appendectomy-enriched and depleted bacteria species including Bacteroides fragilis, Bacteroides vulgatus, Veillonella dispar, Enterococcus hirae, Lachnospiraceae bacterium Choco86, Blautia sp. SC05B48) was shown by using targeted qPCR in appendectomy cases and control subjects.