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. 2023 Feb 10;14(2):102. doi: 10.1038/s41419-023-05637-y

Fig. 6. TRAF4 knockout combined with irradiation inhibits tumor growth in vivo.

Fig. 6

A-C. TRAF4 reintroduction into TRAF4-null HT29 cells rescues tumorigenesis under IR treatment (2 Gy/twice per week). TRAF4 WT or TRAF4 C18A mutant was reintroduced into TRAF4 null-HT29 cells and subjected to in vivo tumorigenesis. (n = 5 mice per group). Tumor growth curves (A) were recorded, tumor tissues were subjected to H&E (B) and IHC staining (C). For C, left, representative image; right, qualification. Scale bar, 25 μm. ***p < 0.001. D–F. Introduction of Bcl-xL into TRAF4-null HT29 cells rescues tumorigenesis under IR treatment (2 Gy/twice per week). Bcl-xL was introduced into TRAF4 knockout-HT29 cells and subjected to in vivo tumorigenesis. (n = 5 mice per group). Tumor growth curves (D) were recorded, tumor tissues were subjected to H&E (E) and IHC staining (F). For F, left, representative image; middle and right, qualification for Ki67 (middle) and cleaved-caspase 3 (right). Scale bar, 25 μm.***p < 0.001. **p < 0.01. TNF receptor-associated factor 4, IR ionizing radiation, H&E hematoxylin and eosin, IHC immunohistochemistry.