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. 2023 Feb 10;9:56. doi: 10.1038/s41420-023-01355-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Immunohistochemistry (IHC) was used to analyze the clinicopathologically collected HCC tissues (n = 100), and the results showed that the expression of four ERS marker proteins was positively correlated with NAT10. B Using the TCGA database, it was found that the correlation analysis between NAT10 and ERS marker proteins in HCC tissues was positive. C CCK-8 method was used to detect the inhibition rate of Lenvatinib and Remodelin on the two cell lines. D qPCR was used to detect the knockdown effect of siRNA on the expression of NAT10 in the two cell lines, and the results showed that Huh-7 alone could knock down the expression of NAT10 by more than 70%. E Western blot showed that Remodelin, a NAT10 inhibitor, significantly inhibited the expression of NAT10 in Huh-7 and hep3B cell lines. F huh-7 cells were treated with NAT10-siRNA for 24 h. Total cell extracts were prepared and analyzed by western blotting using antibodies against GRP78, ATF6, IRE-1, PERK, and GAPDH. G huh-7 and Hep3B cells were treated with Remodelin (20 μmmol/L) for 24 h. Total cell extracts were prepared and analyzed by western blotting with antibodies to GRP78, atf-6, ire-1, PERK, and GAPDH. H The percentage of NAT10(green) in HuH-7 cells increased 24 h after TM (3 μmmol/L) induction, and decreased 24 h after siRNA and Remodelin treatment. I The percentage of NAT10(green) in hep3B cells increased 24 h after TM (3 μmmol/L) induction, and decreased 24 h after siRNA and Remodelin treatment. J After 24 h of TM (3 μmmol/L) induction, the percentage of GRP78(green) in Huh-7 cells increased, and after 24 h of TM induction, the percentage of GRP78(green) in HuH-7 cells decreased after 24 h of siRNA and Remodelin treatment. Each experiment was conducted in triplicate. Data were shown as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.