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. 2023 Feb 10;9:56. doi: 10.1038/s41420-023-01355-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — After 24 h of TM (3 μmmol/L) induction, after siRNA-NAT10 or Remodelin treatment, the migration and invasion of Huh-7 (A) and Hep3B cells (B) were analyzed by transwell, scale = 200 μm. C Similarly, the migration of Huh-7 and Hep3B cells was analyzed by wound healing assay (scale = 100 μm). The effect of siRNA-NAT10 (D) and its inhibitor Remodelin (E) on cell cycle arrest (n = 3) was analyzed by flow cytometry. Huh-7 and hep3B cells were treated with different concentrations of Remodelin for 48 h to conduct cell cycle arrest assay. F Western blot showed the protein levels of CDK2, CyclinA, PCNA, and other related cell cycle genes in Huh-7 and hep3B cells after NAT10 knockdown or inhibition. All cells were induced by TM 24 h in advance, and each experiment was conducted in three times. Data were shown as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.