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. 2023 Feb 10;8:63. doi: 10.1038/s41392-023-01316-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — YTHDF2 O-GlcNAcylation is enhanced upon HBV infection. a Immunoblots quantitative analysis of YTHDF2 expression in HBV-associated HCC tumors (n = 48), P < 0.001. b Analysis of YTHDF2 O-GlcNAcylation in HBV-associated HCC tumors (n = 42) by succinylated wheat germ agglutinin (sWGA) pull-down assays. YTHDF2 O-GlcNAcylation levels were quantified, P < 0.01. c Analysis of YTHDF2 O-GlcNAcylation in HBV-transgenic mice (n = 4), normal C57 mice were used as control. d–g YTHDF2 Immunoprecipitation (IP) with anti-Flag M2 agarose beads in hepatoma cells transfected with Flag-YTHDF2 or a vector control. Specifically, HepAD38 cells were cultured without tetracycline (tet) (d), HepG2-NTCP cells were infected with HBV viruses (e), HepG2 cells (f) and Huh7 cells (g) were infected with AdHBV1.3 (named as HepG2-HBV1.3 and Huh7-HBV1.3) and treated with 25 μΜ Thiamet G (TMG) for 12 h. h–k sWGA pull-down assays were performed in HepG2-NTCP cells infected with HBV viruses (h), HepG2-HBV1.3 cells (i, j) and Huh7-HBV1.3 cells (k) treated with 25 μΜ TMG or 20 μΜ OSMI-1 for 12 h. Western blotting was determined by anti-YTHDF2. All the presented input was adjusted to a similar level for the following IP or sWGA-binding assay