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. 2023 Feb 10;8:63. doi: 10.1038/s41392-023-01316-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — O-GlcNAcylation of YTHDF2 promotes hepatoma cell proliferation, invasion and migration in vitro and in vivo. Cells were transfected with YTHDF2 shRNA lentiviral vector to induce endogenous YTHDF2 knockdown, and subsequently infected with adenoviruses expressing Flag-YTHDF2 (WT or S263A). All hepatoma cells were infected with AdHBV1.3. a–c Proliferation ability of HepG2-HBV1.3 cells (a), Huh7-HBV1.3 cells (b) and PLC/PRF/5-HBV1.3 cells (c) was detected by CCK-8 assay as indicated (n = 3, performed in triplicate). d, e Colony formation capacity of HepG2-HBV1.3 cells (d) and Huh7-HBV1.3 cells (e) treated as indicated (n = 3, performed in triplicate). f, g Cell invasion capacity of Huh7-HBV1.3 cells (f) and PLC/PRF/5-HBV1.3 cells (g) was measured by transwell assay as indicated (n = 3, performed in triplicate, bar = 100 μm). h, i Cell migration capacity of Huh7-HBV1.3 cells (h) and PLC/PRF/5-HBV1.3 cells (i) was measured by wound-healing assay as indicated (n = 3, performed in triplicate, bar = 200 μm). j–l MHCC-97H cells were treated as indicated and subcutaneously injected into nude mice (n = 6 per group). j Representative appearance of subcutaneous implantation tumors. k and l Tumor volume (k) and tumor weight (l) of implantation tumors. Data are represented as mean ± SD. One-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001