Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 10;6:164. doi: 10.1038/s42003-023-04543-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Scheme of the self-organizing culture. b Bright-field view of iPSC-S17-derived cell aggregate containing retinal tissue on days 14, 42, 91, and 180 (upper). Scale bar in bright-field view: 100 µm. Immunostaining of iPSC-S17-derived retinal tissue on days 14, 42, 91, and 180 (middle and lower). Crx (green) and Chx10 (red) in middle panels. Pax6 (green) and Recoverin (red) in lower panels. Blue: nuclear staining with DAPI. Scale bar in immunostaining: 20 µm. c Immunostaining of iPSC-S17-derived retinal tissue on day 180. RXRG (green) and NRL (red) in upper-left panel. Lhx2 (red) in upper-right panel. CtBP2 (green) and Recoverin (red) in lower-left panel. Cone-arrestin (green) in lower-right panel. Blue: nuclear staining with DAPI. Scale bar: 20 µm. d Gene expression analysis in iPSC-S17-derived cell aggregates containing retinal tissue on days 0, 6, 14, 45, 60, and 80. In each replicate, RNA was extracted from 48 aggregates. mRNA levels were determined by qPCR analysis. Relative mRNA expression was determined by the delta-delta Ct method with GAPDH as an endogenous control. Data are presented as mean ± SE (n = 4 per time point). e Bright-field view of iPSC-S17-derived retinal sheet. Scale bar: 100 µm. f Immunostaining of iPSC-S17-derived retinal sheet on day 87. Crx (green) and Chx10 (red) in left panel. Rx (green) and Recoverin (red) in right panel. Blue: nuclear staining with DAPI. Scale bar: 100 µm.