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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Hepatology. 2022 Sep 3;78(5):1478–1491. doi: 10.1002/hep.32719

Figure 4. AAV-mediated CLCF1 overexpression in the liver ameliorates NASH pathologies in mice fed NASH diet.

Figure 4.

A) A schematic outline of study design.

B) Plasma ALT and AST levels in mice transduced with AAV-GFP (n=11) or AAV-CLCF1 (n=9) after 10 weeks of NASH-diet feeding.

C) Liver histology from transduced mice (H&E, left; Sirius Red staining, right).

D) Immunofluorescence staining of Desmin and GPNMB. Scale bar = 100μm.

E) qPCR analysis of hepatic genes involved in cytokine signaling.

F) Immunoblots of total liver lysates from transduced mice.

G) qPCR analysis of genes involved in liver fibrosis, vascular endothelium, inflammation, and hepatic metabolism in transduced mice.

Data in B), D) and F) represent mean ± SEM; two-tailed unpaired Student’s t-test.