Skip to main content
. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Hepatology. 2022 Sep 3;78(5):1478–1491. doi: 10.1002/hep.32719

Figure 6. Hepatocyte-specific deletion of LIFR accelerates NASH progression.

Figure 6.

A) A diagram of Cas9-mediated Lifr deletion and study design. Albumin Cre negative/Rosa26-FSF-Cas9 (control) and Cre+/Rosa26-FSF-Cas9 (HepKO) littermates were transduced with AAV-sgRNAs targeting Lifr via tail vein injection. Transduced mice were subjected to NASH diet feeding for nine weeks.

B) Metabolic and NASH parameters of control (n=6) and HepKO (n=8) mice.

C) Liver appearance and histology in control and HepKO mice following ten weeks of NASH diet feeding.

D) Desmin immunofluorescence staining. Scale bar = 100μm.

E) Immunoblots of liver lysates from control and HepKO mice fed NASH diet.

F) qPCR analysis of hepatic gene expression.

Data in B) and F) represent mean ± SEM; two-tailed unpaired Student’s t-test.