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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Int Forum Allergy Rhinol. 2022 Aug 23;13(3):230–241. doi: 10.1002/alr.23073

FIGURE 1.

FIGURE 1

(A) Three genetic constructs combined in mouse model through breeding. (B) Schematic diagram of model mechanism. Cre recombinase driven by the OMP promoter in mature olfactory neurons removes lox-stop, allowing expression of the reverse tetracycline transactivator (rtTA). When doxycycline is administered in the mouse food, rtTA is allosterically activated and can bind the tet-response element (TRE) promoter, resulting in IL-13 expression in mature olfactory neurons. (C) Demonstration of specificity of the OMP-cre/ROSA26-rtTA in the olfactory epithelium. Doxycycline induces GFP expression in olfactory neurons, when a mouse line with a TRE-GFP transgene is bred into the model. (D) IL-13 is not expressed in mice not fed doxycycline but is detectable in nasal lavage fluid after doxycycline induction