| Component | Volume per sample (μL) | Final concentration in 40 μL reaction |
|---|---|---|
| Neutralized Allyl SAM (∼9 mM) | 12 μL | 2.7 mM |
| SUPERase•In RNase Inhibitor (20 U/μL) | 4 μL | 2 U/μL |
| 10× MjDim1 Reaction Buffer | 4 μL | 1× |
| MjDim1 (1.2–2 mM) | 8 μL | 0.4 mM |
| Total | (12 μL) + 28 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.