| Component | Volume per sample (μL) | Final concentration in 50 μL reaction |
|---|---|---|
| NEBNext Ultra II Q5 Master Mix (2×) | 25 μL | 1× |
| NEBNext Unique Dual Index Primer for Illumina (10 μM) | 10 μL | 2 μM |
| cDNA | 15 μL | |
| Total | 50 μL |
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