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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Exp Eye Res. 2022 Dec 23;227:109358. doi: 10.1016/j.exer.2022.109358

Figure 2. The second cryaa null mutant line generated by deleting the start codon and proximal promoter region produced variable lens phenotypes.

Figure 2.

Two gRNAs were designed to remove approximately 860 base pairs of the cryaa gene (A). This large deletion could be easily identified using PCR amplification with primers flanking the gRNA target regions (B). Some cryaa null embryos showed visually normal lenses by DIC imaging (C, compare to wild type lens in Fig. 1D). Typical phenotypes in abnormal lenses included roughness in central primary fiber cells (d) and general disorganization of central fiber cells (e), as seen in our single gRNA null mutant (Fig. 1). Gaps or roughness between peripheral fiber cells were also common (D and E: arrows). Some lenses combined several of these phenotypes (F). We also observed lenses with severe boundaries between fiber cells (G: arrows) and pitting (H). Lenses shown are either 3 or 4 dpf and arranged with retina to the left. Scale bars are 20 microns. Matching embryos for lenses E and F are shown to indicate a lack of general morphological defects (E’ and F’).