Figure 4. Prevalence of different lens abnormalities in wild-type, α-crystallin mutant and cryaa gRNA-injected larvae at 3 and 4 dpf.

Each graph shows the proportion of larvae with that defect type. Larvae examined at 3 and 4 dpf were wild-type (n= 36/38), offspring of homozygous mutants for cryaa (n=41/35), cryaba (n=40/34) and cryabb (n=38/28), and embryos injected with a four-gRNA mix targeting the cryaa gene (crispants; n=27/16). Asterisks indicate statistically significant differences from age-matched wild-type larvae (“N-1” Chi-squared test).