Figure 7. Expression of each α-crystallin gene assayed by qPCR in whole bodies of wild-type and mutant larvae at 7 dpf.

Box and whisker plots show delta Cq values generated with primers for cryaa (A), cryaba (B) and cryabb (C) in three biological replicates of the genotypes indicated on the x-axes. Y-axes are inverted, as lower delta Cq values indicate greater expression. Asterisks indicate statistically significant differences based on ANOVA with Tukey’s post-hoc test (p value < 0.05).