Table 2.
Effect of cryoprotectant on particle size, PI, ZP, EE and DL measured after reconstitution in double distilled water.
| Process Optimized | Composition (% w/v) | Trehalose (% w/v) | Particle Size (nm) | PI | ZP (−mV) | EE (%) | DL (%) |
|---|---|---|---|---|---|---|---|
| HT: 5 min ST: 10 min |
SC: 2.5 LC: 5 DC: 1 |
0 | 376.6 ± 4.58 | 0.427 ± 0.028 | 23.5 ± 0.32 | Not detected | Not detected |
| 2.5 | 287.3 ± 2.98 | 0.317 ± 0.042 | 26.4 ± 0.41 | 57.46 ± 4.73 | 0.62 ± 0.04 | ||
| 5 | 119.5 ± 2.17 | 0.124 ± 0.012 | 34.6 ± 0.32 | 71.43 ± 1.53 | 0.77 ± 0.03 | ||
| 7.5 | 169.4 ± 3.08 | 0.236 ± 0.052 | 33.9 ± 0.18 | 56.29 ± 3.81 | 0.69 ± 0.04 | ||
| 10 | 197.2 ± 3.81 | 0.257 ± 0.032 | 31.2 ± 0.46 | 55.37 ± 5.26 | 0.67 ± 0.05 |