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. 2023 Feb 1;15(3):745. doi: 10.3390/nu15030745

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RLE regulates production of ROS in the bone tissues of the OVX rats. (A) SOD, (B) NO, and (C) MDA levels in rat serum. (D) Images of immunofluorescence staining DHE. Representative femur sections for NOX-1 and 4HNE by immunohistochemistry (IHC). (E) Quantitative analysis of DHE fluorescence intensity. (F) NOX-1- and (G) 4HNE-positive cells were counted and quantified relatively. (H) Quantitative analysis of MitoSOX fluorescence intensity. Data are shown as mean ± SEM. (n = 10, ^# p < 0.05 vs. sham; * p < 0.05 vs. OVX). Sham, sham-operated group; OVX, ovariectomy; RLE, Ramie leaf extract; SOD, superoxide dismutase; NO, nitric oxide; MDA, malondialdehyde, 4HNE; 4-hydroxynonenal.