Skip to main content
. 2023 Feb 1;15(3):745. doi: 10.3390/nu15030745

Figure 7.

Figure 7

RLE inhibits the RNAKL-induced osteoclast formation in RAW264.7 cells. (A) Representative TRAP-stained images (Scale bar = 200 μm). (B) Quantitative analysis of TRAP-positive cells. (C) Expression of osteoclast-specific genes measured by RT-PCR. (D) Quantification of gene expression. (E) Immunoblotting using antibodies against NFATc1, c-fos, and β-actin and (F) respective quantification analysis. Data are shown as mean ± SEM. (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. RANKL) RLE, Ramie leaf extract; NAC, N-acetyl cysteine; MMP, matrix metallopeptidase; TRAP, tartrate-resistant acid phosphatase; cal-R, Calreticulin.