Figure 2.

The bacterial stimulus promotes the growth of colorectal cancer cells over time. In the outer inlets of the device, approximately 1000 bacterial cells were loaded. About 40,000 HCT 116 cells labeled with a red fluorescent protein (RFP) were injected into the middle inlet. Cells were cultured for 24 hrs with Bacillus or lipopolysaccharide (LPS; 10 ng/mL). Then microchannels were washed thoroughly to remove non-adherent HCT 116 cells and extra bacterial cells. Media was fed at a 5 µL/min flow rate. These experiments were performed without hypoxic conditions. A representative image for each time point is shown.