Figure 3.

The effect of Bacillus or lipopolysaccharide (LPS; 10 ng/mL) on the growth of colorectal cancer cells (HCT 116) over time is quantified daily by imaging two to three independent areas of a cell culture chamber. The number of cells in images was counted using Image J. The data represents the average quantification with a standard error of 4–5 images as cell counts (A) and area covered by cells (B). These experiments were performed without hypoxic conditions. Student t-test was used to calculate the p-value. * p < 0.05 and ** p < 0.01.