Figure 5.

Lactisole increases denatonium induced Ca²⁺ release. (a) Lactisole (10 mM, 1 h pretreatment) greatly increased denatonium induced Ca²⁺ release, doubled the percentage of cells that responded to treatment with denatonium, doubled the peak Ca²⁺ release per cell, and reduced the time to peak Ca²⁺ release by 1.5 min. (b) Denatonium-induced Ca²⁺ release was unaffected by glucose concentrations. (c) Denatonium-induced Ca²⁺ release is unaffected by sweet taste receptor agonist sucralose. (d) Lactisole increases Ca²⁺ release from various concentrations of denatonium. (e) The 20 mM of lactisole has an optimal effect on denatonium induced Ca²⁺ signaling pathways. Bar graphs of only 2 comparisons were analyzed by Student’s t-test, bar graphs containing >2 data points were analyzed via ANOVA using (c) Tukey’s post-test for multiple comparisons, (d) Sidak’s post-test for paired comparisons, or (e) Dunnett’s post-test for comparison with control lacking lactisole: ** p < 0.01, *** p < 0.001, **** p < 0.0001, “n.s.” represents no significance.