Figure 2.

Biofilm populations (log₁₀ CFU/cm²) found in the wells of the 96-well microtiter plates for the three bacterial strains (S. Typhimurium, L. monocytogenes, and Y. enterocolitica) before and after disinfection with the T. capitatus EO. For each bacterial strain, the EO was tested in five different concentrations, two-fold dilutions ranging from 0.39 to 6.25% v/v for S. Typhimurium and L. monocytogenes strains, and from 0.25 to 4.00% v/v for Y. enterocolitica strain. The biofilm population of the negative disinfection control (NC; sterile distilled water also containing 10% v/v ethanol) is also shown. The bars represent the mean values ± standard deviations. Mean values followed by different superscript uppercase letters (A–F) differ significantly (p < 0.05). For each bacterial strain, the MBEC of the EO (resulting in more than six log reductions of its biofilm population with respect to that of the NC) is indicated with the vertical arrow.