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. 2023 Jan 25;15(3):613. doi: 10.3390/nu15030613

Figure 2.

Figure 2

Inhibitory effects of EECM on intracellular lipid accumulation, adipogenesis, and lipogenesis in 3T3-L1 cells. The 3T3-L1 cells were cultured in the growth medium (GM) for four days. After the cells reached confluency, they were cultured with or without methylisobutylxanthine, dexamethasone, and insulin (MDI) and various concentrations of EECM. (A) Relative microscopic images of Oil-Red-O-stained 3T3-L1 cells. (B) Quantitative lipid accumulation ratio. After differentiation, total protein was extracted and detected by via Western blotting analysis. Expression levels of (C) AMPKα pathway proteins and (D) lipogenic and (E) adipogenic transcription factors. Values are represented as the mean ± standard deviation (SD). # p < 0.05 vs. the GM group; *** p < 0.001 vs. the differentiation medium (DM) group.