Figure 3.

High-grade TB of undifferentiated carcinoma cases. (A) H&E staining showed small clusters tumor cells and single cells invading the stroma without glandular formation. (B) 3D image of undifferentiated carcinoma (confocal laser scanning microscope). The tumor was composed of sheet of neoplastic cells. Toward the periphery, the tumor became discohesive, with neoplastic cells forming small clusters of cells and eventually single cells. (C) Detached clusters of cells and single cells were noted (surface-rendering image). (D) High magnification of undifferentiated carcinoma at the periphery of tumor in the region shown in C (surface-rendering image). Detached single cells (arrows) were noted (scale bar, 100 μm).