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. 2023 Jan 24;28(3):1165. doi: 10.3390/molecules28031165

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Five-step protocol for nuclear magnetic relaxation dispersion (NMRD) study, combining the high field magnetisation inversion recovery method (B₀ = 16.4 T) (step 1) with fast field cycling in the variable magnetic field B_rel (4 mT < B_rel ≤ 16.4 T, step 2), remaining at B_rel for a variable time interval τ (step 3) and returning to B₀ (step 4). The upper line shows radiofrequency (RF) pulses (at steps 1 and 5) for free induction decay (FID) detection synchronised with the mechanical shuttling of the NMR sample along the warm bore of the superconducting magnet of the NMR spectrometer.