Figure 3.
(A) MTT assay performed on HGFs treated for 24 h, 48 h, and 72 h with SS diluted at 1:40, 1:60, and 1:80 within the culture medium. Metabolic activity was normalized to control cells treated with DMEM culture medium (CTRL1). The most representative of three separate experiments is shown. Data are presented as the mean ± standard deviation. * vs. CTRL 48 h p = 0.0175; ° vs. CTRL 48 h p = 0.0307; **** vs. CTRL 72 h p < 0.0001; ** vs. CTRL 72 h p = 0.0096. (B) Cytotoxicity assay (LDH assay) of HGFs treated for 24 h and 48 h with SS diluted at 1:40 and 1:80 within the culture medium. The most representative of three separate experiments is shown. Data are presented as the mean ± standard deviation; LDH leakage is LDH release/MTT optical density (OD) values. ** vs. CTRL p = 0.0054; °° vs. CTRL p = 0.0038.
